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KMID : 0545119980080020165
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Journal of Microbiology and Biotechnology
1998 Volume.8 No. 2 p.165 ~ p.170
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On-off Dewatering Control for Lipase-catalyzed Synthesis of n-Butyl Oleate in n-Hexane by Tubular Type Pervaporation System
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Kwon Seok-Joon
Rhee Joon-Shick
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Abstract
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Lipase-catalyzed esterification of n-butyl oleate was carried out in n-hexane as a model reaction. The optimal activity of Candida rugosa lipase was shown in a water activity (a_w) range of 0.52 to 0.65 at 30℃. The water produced from the esterification was removed by a tubular type pervaporation system. The rate of ester formed from the enzymatic esterification was allowed to be the same as the rate of water removal by maintaining an optimal a_w of the reaction system using an on-off dewatering control device. The reaction rate and yield with a_w control were increased two folds higher than the respective values for the uncontrolled reaction.
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